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RNA Sequencing Reveals Widespread Transcription of Natural Antisense RNAs in Entamoeba Species.
- Source :
-
Microorganisms [Microorganisms] 2022 Feb 08; Vol. 10 (2). Date of Electronic Publication: 2022 Feb 08. - Publication Year :
- 2022
-
Abstract
- Entamoeba is a genus of Amoebozoa that includes the intestine-colonizing pathogenic species Entamoeba histolytica . To understand the basis of gene regulation in E. histolytica from an evolutionary perspective, we have profiled the transcriptomes of its closely related species E. dispar , E. moshkovskii and E. invadens. Genome-wide identification of transcription start sites (TSS) and polyadenylation sites (PAS) revealed the similarities and differences of their gene regulatory sequences. In particular, we found the widespread initiation of antisense transcription from within the gene coding sequences is a common feature among all Entamoeba species. Interestingly, we observed the enrichment of antisense transcription in genes involved in several processes that are common to species infecting the human intestine, e.g., the metabolism of phospholipids. These results suggest a potentially conserved and compact gene regulatory system in Entamoeba .
Details
- Language :
- English
- ISSN :
- 2076-2607
- Volume :
- 10
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microorganisms
- Publication Type :
- Academic Journal
- Accession number :
- 35208849
- Full Text :
- https://doi.org/10.3390/microorganisms10020396