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Effects of two polysaccharides from Lepidium meyenii (maca) on intestinal immunity and inflammation in vitro .

Authors :
Chen W
Zhu X
Wang L
Xin X
Zhang M
Source :
Food & function [Food Funct] 2022 Mar 21; Vol. 13 (6), pp. 3441-3452. Date of Electronic Publication: 2022 Mar 21.
Publication Year :
2022

Abstract

In our previous studies, two polysaccharides (MC-1 and MC-2) were identified in the roots of maca ( Lepidium meyenii ). In this study, the effects of these two polysaccharides on intestinal immunity and inflammation were assessed using intestinal epithelial cells, i.e. , Caco-2 cells. The levels of cytokines and tight junction proteins were measured using ELISA and qPCR, respectively. The results showed that MC-1 and MC-2 were difficult to be absorbed in the Caco-2 cell monolayer, but promoted the secretion of IL-8, IL-10, IL-12, and INF-γ in the Caco-2 cells, and the secretion of TNF-α, IL-6, and NO in macrophages (RAW264.7 cells) via the Caco-2 cell monolayer. Additionally, both polysaccharides inhibited damage induced by lipopolysaccharides to the densification of the Caco-2 monolayer membrane by regulating the expressions of TLR4, ZO-1, and occludin. Furthermore, the secretion of inflammatory factors such as TNF-α, IL-8, and INF-γ was inhibited, and the secretion of the anti-inflammatory factor, IL-10, increased. The results indicated that MC-1 and MC-2 can activate intestinal immunity and alleviate intestinal inflammation in vitro .

Details

Language :
English
ISSN :
2042-650X
Volume :
13
Issue :
6
Database :
MEDLINE
Journal :
Food & function
Publication Type :
Academic Journal
Accession number :
35244109
Full Text :
https://doi.org/10.1039/d1fo02659c