Back to Search
Start Over
Ex-vivo mucolytic and anti-inflammatory activity of BromAc in tracheal aspirates from COVID-19.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2022 Apr; Vol. 148, pp. 112753. Date of Electronic Publication: 2022 Feb 25. - Publication Year :
- 2022
-
Abstract
- COVID-19 is a lethal disease caused by the pandemic SARS-CoV-2, which continues to be a public health threat. COVID-19 is principally a respiratory disease and is often associated with sputum retention and cytokine storm, for which there are limited therapeutic options. In this regard, we evaluated the use of BromAc®, a combination of Bromelain and Acetylcysteine (NAC). Both drugs present mucolytic effect and have been studied to treat COVID-19. Therefore, we sought to examine the mucolytic and anti-inflammatory effect of BromAc® in tracheal aspirate samples from critically ill COVID-19 patients requiring mechanical ventilation.<br />Method: Tracheal aspirate samples from COVID-19 patients were collected following next of kin consent and mucolysis, rheometry and cytokine analysis using Luminex kit was performed.<br />Results: BromAc® displayed a robust mucolytic effect in a dose dependent manner on COVID-19 sputum ex vivo. BromAc® showed anti-inflammatory activity, reducing the action of cytokine storm, chemokines including MIP-1alpha, CXCL8, MIP-1b, MCP-1 and IP-10, and regulatory cytokines IL-5, IL-10, IL-13 IL-1Ra and total reduction for IL-9 compared to NAC alone and control. BromAc® acted on IL-6, demonstrating a reduction in G-CSF and VEGF-D at concentrations of 125 and 250 µg.<br />Conclusion: These results indicate robust mucolytic and anti-inflammatory effect of BromAc® ex vivo in tracheal aspirates from critically ill COVID-19 patients, indicating its potential to be further assessed as pharmacological treatment for COVID-19.<br /> (Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Acetylcysteine administration & dosage
Adolescent
Adult
Aged
Aged, 80 and over
Anti-Inflammatory Agents administration & dosage
Anti-Inflammatory Agents pharmacology
Bromelains administration & dosage
Cytokine Release Syndrome pathology
Dose-Response Relationship, Drug
Down-Regulation
Drug Combinations
Expectorants pharmacology
Female
Humans
Inflammation Mediators metabolism
Male
Middle Aged
Respiration, Artificial
Rheology
SARS-CoV-2
Trachea pathology
Young Adult
Acetylcysteine pharmacology
Bromelains pharmacology
COVID-19 pathology
Chemokines drug effects
Cytokines drug effects
Sputum cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 148
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 35272139
- Full Text :
- https://doi.org/10.1016/j.biopha.2022.112753