ZIKV B-cell epitopes for immunodiagnostic tests.

Authors :: Kubiszeski JR
da Silva DJF
Menezes GL
da Silva RA
Zini N
Nogueira ML
Pena LJ
Piccoli JP
Cilli EM
da Silva JM
Bronzoni RVM
Source :: Journal of immunological methods [J Immunol Methods] 2022 May; Vol. 504, pp. 113246. Date of Electronic Publication: 2022 Mar 11.
Publication Year :: 2022
Abstract: The use of serological tests is valuable to diagnose Zika virus (ZIKV) infection and carry out epidemiological surveillance. However, ZIKV serological tests may result in false positives due to cross-reactivity between antibodies against other Flavivirus, especially dengue virus that worldwide disseminated. We used three online tools to predict amino acid sequences of B-cell epitopes. We selected and synthetized two epitopes that showed appropriate features in the molecular dynamic simulation and demonstrated to be suitable for serological assays.<br /> (Copyright © 2022 Elsevier B.V. All rights reserved.)