A novel isoform of hydroxyacyl-CoA dehydrogenase inhibits cell proliferation.

Authors :: Xia G
Gao Y
Wu C
Pan H
Hou J
Su J
Wei S
Gao X
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2022 May 28; Vol. 606, pp. 75-79. Date of Electronic Publication: 2022 Mar 22.
Publication Year :: 2022
Abstract: Hydroxyacyl-CoA dehydrogenase (HADH) catalyzes the third reaction of mitochondrial β-oxidation cascade, while the regulation of its expression and function remains to be elucidated. Using the quantitative translation initiation sequencing (QTI-seq), we have identified that murine Hadh mRNA has two alternative translation start codons. We demonstrated that translation from upstream start codon encodes the mitochondrial isoform of HADH, while translation from downstream start codon produces a short isoform (HADH-S) with predominant nuclear localization. Moreover, overexpression of HADH-S inhibits the proliferation of mouse embryonic fibroblasts. Overall, our results identify a novel isoform of HADH participating in cell proliferation.<br />Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.<br /> (Copyright © 2022 Elsevier Inc. All rights reserved.)