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Urinary Podocyte Count as a Potential Routine Laboratory Test for Glomerular Disease: A Novel Method Using Liquid-Based Cytology and Immunoenzyme Staining.
- Source :
-
Acta cytologica [Acta Cytol] 2022; Vol. 66 (5), pp. 434-440. Date of Electronic Publication: 2022 Mar 29. - Publication Year :
- 2022
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Abstract
- Introduction: This study investigated whether our urinary podocyte detection method using podocalyxin (PDX) and Wilms tumor 1 (WT1) immunoenzyme staining combined with liquid-based cytology can serve as a noninvasive routine laboratory test for glomerular disease.<br />Methods: The presence of PDX- and WT1-positive cells was investigated in 79 patients with glomerular disease and 51 patients with nonglomerular disease.<br />Results: The frequencies and numbers of PDX- and WT1-positive cells were significantly higher in the glomerular disease group than in the nonglomerular disease group. The best cutoffs for PDX- and WT1-positive cell counts for identifying patients with glomerular disease were 3.5 (sensitivity = 67.1% and specificity = 100%) and 1.2 cells/10 mL (sensitivity = 43.0% and specificity = 100%), respectively.<br />Conclusion: Because our urinary podocyte detection method using PDX immunoenzyme staining can be standardized and it detected glomerular disease with high accuracy, it can likely serve as a noninvasive routine laboratory test for various glomerular diseases.<br /> (© 2022 The Author(s). Published by S. Karger AG, Basel.)
Details
- Language :
- English
- ISSN :
- 1938-2650
- Volume :
- 66
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Acta cytologica
- Publication Type :
- Academic Journal
- Accession number :
- 35350010
- Full Text :
- https://doi.org/10.1159/000521675