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Protective effect of remote ischemic postconditioning in rat testes after testicular torsion/detorsion.
- Source :
-
Andrology [Andrology] 2022 Jul; Vol. 10 (5), pp. 973-983. Date of Electronic Publication: 2022 Apr 18. - Publication Year :
- 2022
-
Abstract
- Background: Testicular torsion/detorsion can lead to severe testicular damage. The organ-protective effect of remote ischemic postconditioning (RLIPost) against ischemia/reperfusion injury has been characterized; however, it remains unknown whether RLIPost has a testicular protective effect.<br />Objectives: Here, we tested the hypothesis that RLIPost can protect the testes in a rat model of testicular torsion/detorsion in vivo.<br />Materials and Methods: Male Sprague-Dawley rats were assigned to sham-operated, control, or remote liver and limb ischemic postconditioning-treated groups. Testicular torsion/detorsion was performed by 3 h of testicular torsion (720° clockwise unilateral spermatic cord torsion), followed by 3 h of detorsion. For liver and limb ischemic postconditioning, four cycles of 5 min of liver or limb ischemia with 5-min intermittent reperfusion stimuli were conducted at the onset of testicular reperfusion.<br />Results: Liver and limb ischemic postconditioning significantly ameliorated ipsilateral and contralateral testicular swelling responses, preserved morphological integrity and spermatogenesis and inhibited testicular apoptosis. In addition, RLIPost enhanced the phosphorylation of AKT/ERK1/2/GSK-3β/STAT-3 in the ipsilateral testis while suppressing JNK activation in the ipsilateral and contralateral testes.<br />Discussion and Conclusion: To the best of our knowledge, this study is the first to demonstrate the involvement of RLIPost in an animal model of testicular torsion/detorsion. We showed that RLIPost protects both ipsilateral and contralateral testes against testicular torsion/detorsion in vivo, via at least in part, the RISK and SAFE-mediated signaling pathways.<br /> (© 2022 American Society of Andrology and European Academy of Andrology.)
Details
- Language :
- English
- ISSN :
- 2047-2927
- Volume :
- 10
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Andrology
- Publication Type :
- Academic Journal
- Accession number :
- 35398995
- Full Text :
- https://doi.org/10.1111/andr.13184