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Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7).
- Source :
-
Iranian journal of basic medical sciences [Iran J Basic Med Sci] 2021 Dec; Vol. 24 (12), pp. 1656-1665. - Publication Year :
- 2021
-
Abstract
- Objectives: Inflammation is thought to be the common pathophysiological basis for several disorders. Corilagin is one of the major active compounds which showed broad-spectrum biological and therapeutic activities, such as antitumor, hepatoprotective, anti-oxidant, and anti-inflammatory. This study aimed to evaluate the anti-oxidant and anti-inflammatory activities of corilagin in LPS-induced RAW264.7 cells.<br />Materials and Methods: Anti-oxidant activities were examined by free radical scavenging of H <subscript>2</subscript> O <subscript>2</subscript> , NO, and *OH. The safe concentrations of corilagin on RAW264.7 were determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay on RAW264.7 cell lines. The inflammation cells model was induced with LPS. The anti-inflammatory activities measured IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels using ELISA assay.<br />Results: The results showed that corilagin had a significant inhibition activity dose-dependently in scavenging activities toward H <subscript>2</subscript> O <subscript>2</subscript> , *OH, and NO with IC <subscript>50</subscript> values 76.85 µg/ml, 26.68 µg/ml, and 66.64 µg/ml, respectively. The anti-inflammatory activity of corilagin also showed a significant decrease toward IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels at the highest concentration (75 µM) compared with others concentration (50 and 25 µM) with the highest inhibition activities being 48.09%, 42.37%, 65.69%, 26.47%, 46.88%, 56.22%, 59.99%, respectively ( P <0.05).<br />Conclusion: Corilagin has potential as anti-oxidant and anti-inflammatory in LPS-induced RAW 264.7 cell lines by its ability to scavenge free radical NO, *OH, and H <subscript>2</subscript> O <subscript>2</subscript> and also suppress the production of proinflammatory mediators including COX-2, IL-6, IL-1β, and TNF-α in RAW 264.7 murine macrophage cell lines.
Details
- Language :
- English
- ISSN :
- 2008-3866
- Volume :
- 24
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Iranian journal of basic medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 35432805
- Full Text :
- https://doi.org/10.22038/IJBMS.2021.59348.13174