AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.

Authors :: Eugster A
Bostick ML
Gupta N
Mariotti-Ferrandiz E
Kraus G
Meng W
Soto C
Trück J
Stervbo U
Luning Prak ET
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2453, pp. 261-278.
Publication Year :: 2022
Abstract: The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.<br /> (© 2022. The Author(s).)