Activity-based protein profiling of a surfactin-producing nonribosomal peptide synthetase in Bacillus subtilis .

We present an in vitro and in-cell activity-based protein profiling (ABPP) protocol for endogenous nonribosomal peptide synthetases (NRPSs). This protocol enables the fluorescence labeling and imaging of an endogenous SrfAB-NRPS with high selectivity and sensitivity in the surfactin producer Bacillus subtilis . While we optimized this protocol for use with B. subtilis , the protocol can be applied to Aneurinibacillus migulanus and Escherichia coli . For complete details on the use and execution of this protocol, please refer to Ishikawa et al. (2022).
Competing Interests: The authors declare no competing financial interests.
(© 2022 The Authors.)