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RNA-based next-generation sequencing in non-small-cell lung cancer in a routine setting: an experience from an Italian referral center.
- Source :
-
Personalized medicine [Per Med] 2022 Sep; Vol. 19 (5), pp. 395-401. Date of Electronic Publication: 2022 Jul 08. - Publication Year :
- 2022
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Abstract
- Aim: ALK , ROS1 , NTRK  and RET gene fusions and MET exon 14 skipping alterations represent novel predictive biomarkers for advanced non-small-cell lung cancer (NSCLC). Therefore, testing patients for these genetic variants is crucial for choosing the best selective treatment. Over the last couple of decades, next-generation sequencing (NGS) platforms have emerged as an extremely useful tool for detecting these variants. Materials & methods: In the present study, we report our NGS molecular records produced during a year of diagnostic activity. Results: Overall, our in-house developed NGS workflow successfully analyzed n = 116/131 (88.5%) NSCLC samples. Of these, eight (6.8%) and five (4.3%) out of 116 patients harbored ALK and RET gene rearrangements, respectively: one case harbored ROS1 gene fusion (0.7%). Conclusion: Our results highlight that an RNA-based NGS analysis can reliably detect gene fusion alterations, thereby playing a pivotal role in the management of NSCLC patients.
- Subjects :
- Anaplastic Lymphoma Kinase genetics
High-Throughput Nucleotide Sequencing methods
Humans
Protein-Tyrosine Kinases
Proto-Oncogene Proteins genetics
RNA
Referral and Consultation
Carcinoma, Non-Small-Cell Lung diagnosis
Carcinoma, Non-Small-Cell Lung genetics
Lung Neoplasms diagnosis
Lung Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1744-828X
- Volume :
- 19
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Personalized medicine
- Publication Type :
- Academic Journal
- Accession number :
- 35801400
- Full Text :
- https://doi.org/10.2217/pme-2022-0020