[Relation between the supercoiling of DNA from loach sperm and the environmental temperature].

The effect of environmental temperature changes in the physiological range on DNA supercoiling in the sperm of Misgurnus fossilis L. was studied. Living fishes from the Oka and the Danube and isolated gonads were exposed to temperature changes. In the living fishes, both temperature increase from 4 to 14 degrees C and decrease from 19-21 to 14 degrees resulted in a reversible relaxation of DNA superhelices. Upon decreasing the environmental temperature from 19-21 to 4 degrees C the reversibility of changes in DNA supercoiling was not observed during the next 15 days. In isolated gonads the temperature increase from 4 to 14 degrees C had no effect on the sperm DNA supercoiling. The temperature-dependent changes in the sperm DNA supercoiling were not dependent on the loach population. It is assumed that the effect of changes in environmental temperature on the supercoiling of sperm DNA in vivo plays an important role in the activation of the genome after fertilization.