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Influence of NH 3 and NH 4 + on anaerobic digestion and microbial population structure at increasing total ammonia nitrogen concentrations.

Authors :
Mlinar S
Weig AR
Freitag R
Source :
Bioresource technology [Bioresour Technol] 2022 Oct; Vol. 361, pp. 127638. Date of Electronic Publication: 2022 Jul 16.
Publication Year :
2022

Abstract

Despite the extensive research dedicated to ammonia inhibition, the effect of NH <subscript>3</subscript> and NH <subscript>4</subscript> <superscript>+</superscript> on each anaerobic digestion stage and the associated microorganisms is still not completely understood. In the past, the focus was mainly on methanogenesis and either on NH <subscript>3</subscript> or total ammonia nitrogen (TAN). Here, anaerobic digestion of two defined substrates, namely starch/NH <subscript>4</subscript> Cl and casein, was investigated particularly regarding the effects of different NH <subscript>3</subscript> /NH <subscript>4</subscript> <superscript>+</superscript> ratios on the involved microorganisms. TAN affected bacteria, primarily gram-positive ones, whereas archaea responded largely to the NH <subscript>3</subscript> concentration. These sensitivity differences are attributed to differences in the corresponding cell-membrane structures. A TAN decrease via stripping performed in two full-scale agricultural biogas plants resulted in increased bacterial diversity, with a pronounced increase in the propionate acetogens' abundance. Based on these data, it is suggested that inhibition can be avoided and processes stabilized in biogas plants by adjusting the NH <subscript>3</subscript> /NH <subscript>4</subscript> <superscript>+</superscript> ratio, when feeding nitrogen-rich substrates.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1873-2976
Volume :
361
Database :
MEDLINE
Journal :
Bioresource technology
Publication Type :
Academic Journal
Accession number :
35853595
Full Text :
https://doi.org/10.1016/j.biortech.2022.127638