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Microfluidic Paper-Based Preconcentration and Retrieval for Rapid Ribonucleic Acid Biomarker Detection and Visualization.
- Source :
-
Analytical chemistry [Anal Chem] 2022 Aug 02; Vol. 94 (30), pp. 10764-10772. Date of Electronic Publication: 2022 Jul 20. - Publication Year :
- 2022
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Abstract
- Microfluidic paper-based analytical devices (μPADs) have attracted significant attention in the field of point-of-care (POC) diagnostics. However, the heterogeneous structure of the paper often impairs the limit of detection (LOD) for low-abundance targets when those targets are directly analyzed. One viable solution to bypass this limitation is to elevate the target concentration above the LOD on-site to reach a valid readout. Here, we developed a 3D μPADs preconcentrator (3D-μP <superscript>2</superscript> ) to increase sample concentration by electrokinetic trapping and demonstrated its application in increasing the LOD of a downstream colorimetric assay. The three-dimensional (3D) structure of this device was composed of a loading pad, a vertical fluid path formed by stacked absorbent pads, and an ion-selective membrane of PEDOT:PSS. This novel design facilitates fast preconcentration, high capacity in sample processing, and easy target retrieval. The concentration of an exemplary target, a single-stranded DNA sequence, was increased up to 170-fold within 80 s. The LOD of the colorimetric assay to verify the DNA target was increased 3 orders of magnitude with a preconcentrated sample compared to the control. The device and its analysis equipment used in this study were all cheap and portable. Thus, the 3D-μP <superscript>2</superscript> can be a powerful POC tool for sample pretreatment in resource-limited areas.
- Subjects :
- Biomarkers
Paper
RNA
Microfluidic Analytical Techniques
Microfluidics
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 94
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 35858837
- Full Text :
- https://doi.org/10.1021/acs.analchem.2c01442