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Constitutive expression of JASMONATE RESISTANT 1 induces molecular changes that prime the plants to better withstand drought.
- Source :
-
Plant, cell & environment [Plant Cell Environ] 2022 Oct; Vol. 45 (10), pp. 2906-2922. Date of Electronic Publication: 2022 Aug 03. - Publication Year :
- 2022
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Abstract
- In this study, we investigated Arabidopsis thaliana plants with altered levels of the enzyme JASMONATE RESISTANT 1 (JAR1), which converts jasmonic acid (JA) to jasmonoyl-l-isoleucine (JA-Ile). Analysis of a newly generated overexpression line (35S::JAR1) revealed that constitutively increased JA-Ile production in 35S::JAR1 alters plant development, resulting in stunted growth and delayed flowering. Under drought-stress conditions, 35S::JAR1 plants showed reduced wilting and recovered better from desiccation than the wild type. By contrast, jar1-11 plants with a strong reduction in JA-Ile content were hypersensitive to drought. RNA-sequencing analysis and hormonal profiling of plants under normal and drought conditions provided insights into the molecular reprogramming caused by the alteration in JA-Ile content. Especially 35S::JAR1 plants displayed changes in expression of developmental genes related to growth and flowering. Further transcriptional differences pertained to drought-related adaptive systems, including stomatal density and aperture, but also reactive oxygen species production and detoxification. Analysis of wild type and jar1-11 plants carrying the roGFP-Orp1 sensor support a role of JA-Ile in the alleviation of methyl viologen-induced H <subscript>2</subscript> O <subscript>2</subscript> production. Our data substantiate a role of JA-Ile in abiotic stress response and suggest that JAR1-mediated increase in JA-Ile content primes Arabidopsis towards improved drought stress tolerance.<br /> (© 2022 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)
Details
- Language :
- English
- ISSN :
- 1365-3040
- Volume :
- 45
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Plant, cell & environment
- Publication Type :
- Academic Journal
- Accession number :
- 35864601
- Full Text :
- https://doi.org/10.1111/pce.14402