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Mechanisms underlying microRNA-222-3p modulation of methamphetamine-induced conditioned place preference in the nucleus accumbens in mice.
- Source :
-
Psychopharmacology [Psychopharmacology (Berl)] 2022 Sep; Vol. 239 (9), pp. 2997-3008. Date of Electronic Publication: 2022 Jul 26. - Publication Year :
- 2022
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Abstract
- Rationale: MicroRNA (miRNA) control of post-transcription gene expression in the nucleus accumbens (NAc) has been implicated in methamphetamine (METH) dependence. Conditioned place preference (CPP) is a classical animal procedure that reflects the rewarding effects of addictive drugs. miR-222-3p has been reported to play a key role in various neurological diseases and is strongly associated with alcohol dependence. Nevertheless, the role of miR-222-3p in METH dependence remains unclear.<br />Objective: To explore the molecular mechanisms underlying the role of miR-222-3p in the NAc in METH-induced CPP.<br />Methods: miR-222-3p expression in the NAc of METH-induced CPP mice was detected by quantitative real-time (qPCR). Following adeno-associated virus (AAV)-mediated overexpression or knockdown of miR-222-3p in the NAc, mice were subjected to CPP to investigate the effects of miR-222-3p on METH-induced CPP. Target genes of mir-222-3p were predicted using bioinformatics analysis. Candidate target genes for METH-induced CPP were validated by qPCR.<br />Results: miR-222-3p expression in the NAc was decreased in CPP mice. Overexpression of miR-222-3p in the NAc blunted METH-induced CPP. Ppp3r1, Cdkn1c, Fmr1, and PPARGC1A were identified as target gene transcripts potentially mediating the effects of miR-222-3p on METH-induced CPP.<br />Conclusion: Our results highlight miR-222-3p as a key epigenetic regulator in METH-induced CPP and suggest a potential role for miR-222-3p in the regulation of METH-induced reward-related changes in the brain.<br /> (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Animals
Fragile X Mental Retardation Protein
Mice
Nucleus Accumbens
Amphetamine-Related Disorders metabolism
Central Nervous System Stimulants metabolism
Central Nervous System Stimulants pharmacology
Methamphetamine metabolism
Methamphetamine pharmacology
MicroRNAs genetics
MicroRNAs metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-2072
- Volume :
- 239
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Psychopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 35881147
- Full Text :
- https://doi.org/10.1007/s00213-022-06183-9