Uncovering the effect of polyethyleneimine on methane production in anaerobic digestion of sewage sludge.

The potential effect of polyethyleneimine as a flocculant on anaerobic digestion of sludge was investigated. Polyethyleneimine above 12 g/kg total suspended solids inhibited the entire anaerobic digestion process including solubilization, hydrolysis, acidification, and methanogenesis. The addition of 24 g/kg total suspended solids polyethyleneimine reduced methane production from 167 ± 5 L/kg volatile suspended solids in the control reactor (without polyethyleneimine) to 141 ± 5 L/kg volatile suspended solids. Polyethyleneimine bound to extracellular polymeric substances, thus enhancing sludge agglomeration and hindering the release of organics. Meanwhile, the reduction of cytochrome C impeded electron transport, consequently curbed direct interspecies electron transfer. The adsorption of carbon dioxide by amine groups also hampered methane conversion. This study elucidated the concept that polyethyleneimine reduces mass transfer in anaerobic digestion, providing new insights into the potential behavior of flocculants in sludge treatment.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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