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Cu 2+ -Mediated Aggregation of Gold Nanoparticles as an Optical Probe for the Detection of Endotoxin.
- Source :
-
Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2022 Sep 06; Vol. 38 (35), pp. 10826-10835. Date of Electronic Publication: 2022 Aug 22. - Publication Year :
- 2022
-
Abstract
- Endotoxins or lipopolysaccharides (LPS) present in the outer layer of Gram-negative bacteria (GNB) are responsible for bacterial toxicity. It is an environmental hazard that everyone is exposed to daily to various extents. Due to its potent toxicity, quantitative detection with very high sensitivity is essential in the food, medical, and pharmaceutical industries. Herein, we report an optical nanosensor for the rapid and sensitive detection of LPS and GNB based on the Cu <superscript>2+</superscript> -mediated aggregation of gold nanoparticles (Cu@AuNPs). The sensor detects LPS within a linear range of 20 ag/mL to 20 ng/mL with a lower detection limit of 0.2 ag/mL. The sensor could successfully recover spiked endotoxin in grape juice with a percentage error of ±0.2, confirming its application in the food industry. The sensor could also distinguish Gram-negative bacteria from Gram-positive bacteria, and the selectivity of the Cu@AuNP sensor toward GNB is utilized to detect Escherichia coli in wastewater. The rapid detection of E. coli without any pretreatment is a promising strategy in water analysis.
Details
- Language :
- English
- ISSN :
- 1520-5827
- Volume :
- 38
- Issue :
- 35
- Database :
- MEDLINE
- Journal :
- Langmuir : the ACS journal of surfaces and colloids
- Publication Type :
- Academic Journal
- Accession number :
- 35994084
- Full Text :
- https://doi.org/10.1021/acs.langmuir.2c01436