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Transcriptionally amplified synthesis of fluorogenic RNA aptamers for label-free DNA glycosylase assay.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2022 Sep 13; Vol. 58 (73), pp. 10229-10232. Date of Electronic Publication: 2022 Sep 13. - Publication Year :
- 2022
-
Abstract
- We demonstrate for the first time the utilization of fluorogenic RNA aptamers for label-free uracil-DNA glycosylase (UDG) assay. Through rationally engineering the transcription machine with dU substitution, this assay requires only a single probe to simultaneously sense and amplify the UDG signal, achieving a low detection limit of 6.3 × 10 <superscript>-6</superscript> U mL <superscript>-1</superscript> . Moreover, it can be applied for screening UDG inhibitors and measuring endogenous UDG activity in different cells.
- Subjects :
- DNA Repair
Uracil-DNA Glycosidase metabolism
Aptamers, Nucleotide
Subjects
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 58
- Issue :
- 73
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 36004508
- Full Text :
- https://doi.org/10.1039/d2cc03628b