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Pilot study of fluorescence imitating brightfield imaging for rapid, slide-free dermatopathology.
- Source :
-
Journal of cutaneous pathology [J Cutan Pathol] 2022 Dec; Vol. 49 (12), pp. 1060-1066. Date of Electronic Publication: 2022 Aug 31. - Publication Year :
- 2022
-
Abstract
- Background: Fluorescence imitating brightfield imaging (FIBI) is a novel alternative microscopy method that can image freshly excised, non-sectioned tissue. We examine its potential utility in dermatopathology by examining readily available specimens embedded in paraffin blocks.<br />Methods: Nine skin samples embedded in paraffin blocks were superficially deparaffinized using xylene and ethanol and stained with H&E. FIBI captured tissue surface histopathology images using simple microscope optics and a color camera. We then applied deep-learning-based models to improve resemblance to standard H&E coloration and contrast. FIBI images were compared with corresponding standard H&E slides and concordance was assessed by two dermatopathologists who numerically scored epidermal and dermal structure appearance and overall diagnostic utility.<br />Results: Dermatopathologist scores indicate that FIBI images are at least equivalent to standard H&E slides for visualizing structures such as epidermal layers, sweat glands, and nerves.<br />Conclusion: Images acquired with FIBI are comparable to traditional H&E-stained slides, suggesting that this rapid, inexpensive, and non-destructive microscopy technique is a conceivable alternative to standard histopathology processes especially for time-sensitive procedures and in settings with limited histopathology resources.<br /> (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Humans
Pilot Projects
Staining and Labeling
Epidermis
Paraffin
Microscopy methods
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0560
- Volume :
- 49
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of cutaneous pathology
- Publication Type :
- Academic Journal
- Accession number :
- 36053830
- Full Text :
- https://doi.org/10.1111/cup.14308