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Identification and functional characterization of C-type lectins and crustins provide new insights into the immune response of Portunus trituberculatus.
- Source :
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Fish & shellfish immunology [Fish Shellfish Immunol] 2022 Oct; Vol. 129, pp. 170-181. Date of Electronic Publication: 2022 Aug 31. - Publication Year :
- 2022
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Abstract
- A meticulous understanding of the immune characteristics of aquaculture animals is the basis for developing precise disease prevention and control strategies. In this study, four novel C-type lectins (PtCTL-5, PtCTL-6, PtCTL-7 and PtCTL-8) including a single carbohydrate-recognition domain (CRD), and four novel crustins (Ptcrustin-1, Ptcrustin-2, Ptcrustin-3 and Ptcrustin-4) with a single whey acidic protein (WAP) domain were identified from the swimming crab Portunus trituberculatus. Tissue distribution analysis indicated that most of the target genes were predominantly expressed in the hepatopancreas in all examined tissues, except for Ptcrustin-1 which were mainly expressed in the gills. Our results showed that the eight genes displayed various transcriptional profiles across different tissues. In hemocytes, the PtCTL-7 responded quickly to Vibrio alginolyticus and exhibited much more strongly up-regulation than other three PtCTLs. The Ptcrustin-1 rapidly responded to V. alginolyticus within 3 h in all the three tested tissues. Furthermore, recombinant proteins of PtCTL-5 and PtCTL-8 were successfully obtained, and both of them displayed bacterial binding activities toward V. alginolyticus, V. harveyi and Staphylococcus aureus, and only showed antibacterial activity against V. harveyi. These findings provided new insights into the diverse immune response of P. trituberculatus and laid theoretical foundations for the development of precise disease prevention and control strategies in P. trituberculatus farming. Moreover, the specific anti-V. harveyi activities exhibited by rPtCTL-5 and rPtCTL-8 suggested their promising application prospects for controlling diseases caused by V. harveyi.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Anti-Bacterial Agents metabolism
Anti-Bacterial Agents pharmacology
Antimicrobial Cationic Peptides chemistry
Aquaculture
Arthropod Proteins chemistry
Base Sequence
Brachyura classification
Brachyura genetics
Carbohydrates isolation & purification
Immunity, Innate genetics
Lectins, C-Type chemistry
Lectins, C-Type immunology
Phylogeny
Recombinant Proteins genetics
Sequence Alignment
Antimicrobial Cationic Peptides immunology
Brachyura immunology
Lectins, C-Type physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 129
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 36057429
- Full Text :
- https://doi.org/10.1016/j.fsi.2022.08.070