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Effect of pre-gelatinization on α-amylase-catalyzed hydrolysis of corn starch under moderate electric field.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2022 Nov 30; Vol. 221, pp. 1335-1344. Date of Electronic Publication: 2022 Sep 07. - Publication Year :
- 2022
-
Abstract
- This study aimed to explore the roles of starch structure in α-amylase-catalyzed hydrolysis under moderate electric field (MEF). Corn starch was gelatinized by controlling the temperature procedure of rapid viscos-analysis, and then the pre-gelatinized starch (3.0 g) was treated by MEF (2.5 and 5 V/cm) in the presence of α-amylase (1.5 mL). Only a slight hydrolysis occurred for native starch, showing minor increases in reducing sugar content (RSC, ∼0.19 mg/mL), slight changes in granular and semicrystalline structure, and decreases in thermostability (the maximum decomposition temperature (T <subscript>max</subscript> ) decreased from 322 to 300 °C). The densely-packed semicrystalline within starch granules was destroyed by pre-gelatinization, thus enhancing the hydrolysis and further decreasing the thermostability, presenting RSC values of 0.63-0.92 mg/mL and T <subscript>max</subscript> of 291-292 °C. Moreover, some special crystals were formed by IEF-induced orientation of hydrolyzed starch chains. Overall, these results confirmed that the semicrystalline structure of starch dominated in MEF-assisted hydrolysis, which could provide guidance for the application of electro-based techniques in starch modification.<br />Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.<br /> (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Subjects :
- Hydrolysis
Zea mays chemistry
Amylases
Catalysis
Starch chemistry
alpha-Amylases
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 221
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 36087753
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2022.09.028