Back to Search
Start Over
Expression level of Sec62 modulates membrane insertion of marginally hydrophobic segments.
- Source :
-
Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2022 Dec 01; Vol. 1864 (12), pp. 184051. Date of Electronic Publication: 2022 Sep 17. - Publication Year :
- 2022
-
Abstract
- In the endoplasmic reticulum (ER) membrane, transmembrane (TM) domain insertion occurs through the Sec61 channel with its auxiliary components, including Sec62. Sec62 interacts with the Sec61 channel and is located on the front side of the Sec61 lateral gate, an entry site for TM domains to the lipid bilayer. Overexpression of Sec62 led to a growth defect in yeast, and we investigated its effects on protein translocation and membrane insertion by pulse labeling of Sec62 client proteins. Our data show that the insertion efficiency of marginally hydrophobic TM segments is reduced upon Sec62 overexpression. This result suggests a potential regulatory role of Sec62 as a gatekeeper of the lateral gate, thereby modulating the insertion threshold of TM segments.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Subjects :
- Endoplasmic Reticulum metabolism
Hydrophobic and Hydrophilic Interactions
Membrane Transport Proteins metabolism
Protein Transport
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Lipid Bilayers metabolism
Saccharomyces cerevisiae Proteins chemistry
Saccharomyces cerevisiae Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-2642
- Volume :
- 1864
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta. Biomembranes
- Publication Type :
- Academic Journal
- Accession number :
- 36122759
- Full Text :
- https://doi.org/10.1016/j.bbamem.2022.184051