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Selenium-Enriched Black Soybean Protein Prevents Benzo( a )pyrene-Induced Pyroptotic Colon Damage and Gut Dysbacteriosis.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2022 Oct 05; Vol. 70 (39), pp. 12629-12640. Date of Electronic Publication: 2022 Sep 21. - Publication Year :
- 2022
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Abstract
- Selenium-enriched black soybean protein (SeBSP) is a kind of high-quality selenium resource with many physiological functions. Benzo( a )pyrene (BaP) is a well-known injurant that widely exists in high-temperature processed food and has been previously found to cause colon injury. In this study, the effects of SeBSP on colonic damage induced by BaP in BALB/C mice were investigated by comparing it with normal black soybean protein (BSP). SeBSP inhibited the BaP-induced reductions on body weight, food intake, and water intake. Moreover, metabolic enzymes, including AhR, CYP1A1, CYP1B1, and GST-P1, that were promoted by BaP were downregulated by SeBSP, reducing oxidative damage caused by BaP in the metabolic process. The classical pyroptosis indexes (i.e., NLRP3, ASC, Caspase-1, GSDMD) and inflammatory factors (i.e., TNF-α, IL-1β, IL-18, iNOS, COX-2) were downregulated by SeBSP in BaP-treated mice, suggesting the benefits of SeBSP in reducing colonic toxicity. Notably, SeBSP enhanced microbial diversity of gut microbiota and increased relative abundances of prebiotic bacteria, for example, Lactobacillus reuteri , Bacteroides thetaiotaomicron , and genera Bifidobacterium, and Blautia , along with the promotion of short-chain fatty acids. Integrative analysis showed strong links between the antioxidant and anti-inflammatory effects of SeBSP and its altered gut microbiota. Collectively, our study demonstrates the pronounced benefits of Se-enriched black soybean in preventing the colonic toxicity of BaP, and such effects could be mediated by gut microbiota.
- Subjects :
- Animals
Anti-Inflammatory Agents pharmacology
Antioxidants metabolism
Caspases metabolism
Colon metabolism
Cyclooxygenase 2 metabolism
Cytochrome P-450 CYP1A1 metabolism
Dysbiosis metabolism
Interleukin-18 metabolism
Mice
Mice, Inbred BALB C
NLR Family, Pyrin Domain-Containing 3 Protein metabolism
Soybean Proteins metabolism
Tumor Necrosis Factor-alpha metabolism
Benzo(a)pyrene metabolism
Selenium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 70
- Issue :
- 39
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 36129345
- Full Text :
- https://doi.org/10.1021/acs.jafc.2c04526