Back to Search Start Over

COVID-19 variants' cross-reactivity on the paper microfluidic particle counting immunoassay.

Authors :
Kim S
Eades C
Yoon JY
Source :
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2022 Nov; Vol. 414 (28), pp. 7957-7965. Date of Electronic Publication: 2022 Sep 21.
Publication Year :
2022

Abstract

SARS-CoV-2 has mutated many times since the onset of the COVID-19 pandemic, and the omicron is currently the most dominant variant. Determining the specific strain of the virus is beneficial in providing proper care and containment of the disease. We have previously reported a novel method of counting the number of particle immunoagglutination on a paper microfluidic chip using a smartphone-based fluorescence microscope. A single-copy-level detection was demonstrated from clinical saline gargle samples. In this work, we further evaluated two different SARS-CoV-2 monoclonal antibodies to spike vs. nucleocapsid antigens for detecting omicron vs. delta and spike vs. nucleocapsid proteins. The SARS-CoV-2 monoclonal antibody to nucleocapsid proteins could distinguish omicron from delta variants and nucleocapsid from spike proteins. However, such distinction could not be found with the monoclonal antibody to spike proteins, despite the numerous mutations found in spike proteins among variants. This result may suggest a clue to the role of nucleocapsid proteins in recognizing different variants.<br /> (© 2022. Springer-Verlag GmbH Germany, part of Springer Nature.)

Details

Language :
English
ISSN :
1618-2650
Volume :
414
Issue :
28
Database :
MEDLINE
Journal :
Analytical and bioanalytical chemistry
Publication Type :
Academic Journal
Accession number :
36131142
Full Text :
https://doi.org/10.1007/s00216-022-04333-8