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Platelet depletion enhances lethal, hemorrhagic and myotoxic activities of Bothrops asper snake venom in a murine model.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 2022 Nov; Vol. 219, pp. 106936. Date of Electronic Publication: 2022 Oct 03. - Publication Year :
- 2022
-
Abstract
- Platelets play key roles in hemostasis, inflammation, immune response, and tissue repair. Although it is known that viperid snake venoms induce thrombocytopenia and platelet hypoaggregation, the roles of these effects in the overall outcome of envenoming are poorly known. This study aimed to assess the effect of platelet depletion on several toxic activities induced by the venom of the Central American viperid snake Bothrops asper in a mouse model. A profound thrombocytopenia was induced in mice by the administration of aspercetin, a C-type lectin-like protein that induces platelet agglutination and drop in platelet counts, while a control group was treated with saline solution instead. Upon envenoming, animals rendered thrombocytopenic developed a higher extent of local and systemic hemorrhage and local myonecrosis, as compared to control envenomed mice. In addition, the median lethal dose (LD <subscript>50</subscript> ), determined by the intraperitoneal route, was significantly lower in thrombocytopenic mice, underscoring a higher toxicity of venom in these conditions. No difference in the value of LD <subscript>50</subscript> between the two groups was observed when using the intravenous route of injection, and no difference was observed in the magnitude and time-course of footpad edema. Skeletal muscle regeneration was assessed 14 days after venom injection in muscle. Both experimental groups showed a similarly poor regeneration, suggesting that platelets do not play a key role in the regenerative process in these experimental conditions. Results indicate that depletion of platelets increases hemorrhagic and myotoxic effects, as well as overall toxicity, of B. asper venom, implying that platelets play a protective hemostatic role in this model of envenoming.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022. Published by Elsevier Ltd.)
- Subjects :
- Mice
Animals
Disease Models, Animal
Saline Solution toxicity
Saline Solution metabolism
Snake Venoms toxicity
Hemorrhage chemically induced
Myotoxicity
Lectins, C-Type metabolism
Bothrops metabolism
Crotalid Venoms pharmacology
Thrombocytopenia chemically induced
Hemostatics toxicity
Hemostatics metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3150
- Volume :
- 219
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 36202178
- Full Text :
- https://doi.org/10.1016/j.toxicon.2022.106936