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Composition and Bioactivity of a Modified Huang-Lian-Jie-Du Decoction.

Authors :: Lin MY
Chen LG
Siao YY
Lin TH
Huang IA
Liu YW
Huang CC
Source :: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2022 Sep 27; Vol. 2022, pp. 2147923. Date of Electronic Publication: 2022 Sep 27 (Print Publication: 2022).
Publication Year :: 2022
Abstract: Background: Epidermal growth factor receptor inhibitors (EGFRIs) and tyrosine kinase inhibitors (TKIs) are key drugs in targeted cancer therapy. However, they may cause skin toxicity. We previously prepared a modified Huang-Lian-Jie-Du (mHLJD) decoction cream using 10 herbs, which effectively alleviated EGFRI/TKI-induced skin toxicity. In the present study, we identified the reference markers of the mHLJD decoction and investigated the anti-inflammatory and antibacterial effects of the mHLJD decoction extract.<br />Methods: We performed high-performance liquid chromatography (HPLC) to determine the composition of the mHLJD decoction. Human epidermoid A431 cells were treated with tumor necrosis factor (TNF)- α to induce inflammation; then, the effects of the mHLJD decoction extract on the cytokine expression were determined using a cytokine array and by performing real-time quantitative polymerase chain reaction (qPCR). The antibacterial effects of the extract were examined using disk diffusion and microdilution assays.<br />Results: HPLC results revealed that the mHLJD decoction primarily consisted of geniposide, berberine chloride, baicalin, coptisine, and palmatine. TNF- α treatment increased the expression of certain cytokines, including IL-1 β , IL-8, M-CSF, and TGF- β 2; however, pretreatment with the mHLJD decoction extract reduced their expression. The qPCR results demonstrated a decreased mRNA expression of IL-8, M-CSF, and TGF- β 2. The antibacterial assay revealed that the extract exerted inhibitory effects on Staphylococcus aureus , forming an inhibition zone at the minimum inhibitory concentrations of 3.125 and 6.25 mg/mL; however, the extract exerted no effects on Escherichia coli and Pseudomonas aeruginosa. Conclusions . We developed an HPLC method to quantify the reference markers of the mHLJD decoction. The bioactivity analysis provided the potential mechanisms underlying the effects of the mHLJD decoction on EGFRI/TKI-induced skin toxicity.<br />Competing Interests: The authors declare that they have no conflicts of interest.<br /> (Copyright © 2022 Mei-Yi Lin et al.)