Electrochemiluminescence resonance energy transfer of MnCO 3 for ultrasensitive amyloid-β protein detection.

A composite material MnCO ₃ /poly(diallyl dimethyl ammonium chloride) (PDDA)/Ag with excellent electrochemiluminescence (ECL) performance and high biocompatibility was prepared by adding MnCO ₃ and PDDA to silver nanoparticles (AgNPs). MnCO ₃ /PDDA/Ag and Au@SiO ₂ NPs were used as ECL donors and acceptors, respectively. Thus, an effective ECL-resonance energy transfer (RET) sensing platform was established. In a potassium persulfate (K ₂ S ₂ O ₈ ) medium, MnCO ₃ exhibited ECL emission with an ECL band appearing at 500-600 nm. In addition, Au@SiO ₂ nanoparticles showed a UV-visible absorption at 450-650 nm. The ECL emission spectra of MnCO ₃ overlapped with the absorption spectra of Au@SiO ₂ NPs. The effective ECL quenching resulted in a good response to the concentration of Aβ ₄₂ in serum samples. The linear range was 5 fg ⋅ mL ^-1 to 100 ng ⋅ mL ^-1 , and the detection limit was 2 fg ⋅ mL ^-1 . The recovery ranged from 97.7% to 104%. The high-efficiency ECL-RET immunosensor has potential application in detecting human serum Aβ ₄₂ and other biomarkers, and can be used for the early screening of diseases.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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