Biosensor Cell-Fit-HD 4D for correlation of single-cell fate and microscale energy deposition in complex ion beams.

Authors :: Schlegel J
Liew H
Rein K
Dzyubachyk O
Debus J
Abdollahi A
Niklas M
Source :: STAR protocols [STAR Protoc] 2022 Oct 29; Vol. 3 (4), pp. 101798. Date of Electronic Publication: 2022 Oct 29 (Print Publication: 2022).
Publication Year :: 2022
Abstract: We present a protocol for the biosensor Cell-Fit-HD <superscript>4D</superscript> . It enables long-term monitoring and correlation of single-cell fate with subcellular-deposited energy of ionizing radiation. Cell fate tracking using widefield time-lapse microscopy is uncoupled in time from confocal ion track imaging. Registration of both image acquisition steps allows precise ion track assignment to cells and correlation with cellular readouts. For complete details on the use and execution of this protocol, please refer to Niklas et al. (2022).<br />Competing Interests: The authors declare no competing interests.<br /> (© 2022 The Author(s).)

Subjects :: Microscopy, Confocal methods
Radiometry methods
Cell Tracking
Linear Energy Transfer
Biosensing Techniques

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