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Analytical Method for Experimental Validation of Computer-Designed Antibody.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2552, pp. 409-433. - Publication Year :
- 2023
-
Abstract
- In the computational design of antibodies, the interaction analysis between target antigen and antibody is an essential process to obtain feedback for validation and optimization of the design. Kinetic and thermodynamic parameters as well as binding affinity (K <subscript>D</subscript> ) allow for a more detailed evaluation and understanding of the molecular recognition. In this chapter, we summarize the conventional experimental methods which can calculate K <subscript>D</subscript> value (ELISA, FP), analyze a binding activity to actual cells (FCM), and evaluate the kinetic and thermodynamic parameters (ITC, SPR, BLI), including high-throughput analysis and a recently developed experimental technique.<br /> (© 2023. Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Enzyme-Linked Immunosorbent Assay
Kinetics
Computers
Antibodies
Antigens
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2552
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 36346606
- Full Text :
- https://doi.org/10.1007/978-1-0716-2609-2_23