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Genetic diversity of Trypanosoma cruzi strains isolated from chronic chagasic patients and non-human hosts in the state of São Paulo, Brazil.

Authors :
Souza TKM
Westphalen EVN
Westphalen SDR
Taniguchi HH
Elias CR
Motoie G
Gava R
Pereira-Chioccola VL
Novaes CTG
Carvalho NB
Bocchi EA
Cruz FDDD
Rocha MC
Shinjo SK
Shikanai-Yasuda MA
Ortiz PA
Teixeira MMG
Tolezano JE
Source :
Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2022 Nov 11; Vol. 117, pp. e220125. Date of Electronic Publication: 2022 Nov 11 (Print Publication: 2022).
Publication Year :
2022

Abstract

Background: Trypanosoma cruzi shows an exuberant genetic diversity. Currently, seven phylogenetic lineages, called discrete typing units (DTUs), are recognised: TcI-TcVI and Tcbat. Despite advances in studies on T. cruzi and its populations, there is no consensus regarding its heterogeneity.<br />Objectives: This study aimed to perform molecular characterisation of T. cruzi strains, isolated in the state of São Paulo, to identify the DTUs involved and evaluate their genetic diversity.<br />Methods: T. cruzi strains were isolated from biological samples of chronic chagasic patients, marsupials and triatomines through culture techniques and subjected to molecular characterisation using the fluorescent fragment length barcoding (FFLB) technique. Subsequently, the results were correlated with complementary information to enable better discrimination between the identified DTUs.<br />Findings: It was possible to identify TcI in two humans and two triatomines; TcII/VI in 19 humans, two marsupials and one triatomine; and TcIII in one human host, an individual that also presented a result for TcI, which indicated the possibility of a mixed infection. Regarding the strains characterised by the TcII/VI profile, the correlation with complementary information allowed to suggest that, in general, these parasite populations indeed correspond to the TcII genotype.<br />Main Conclusions: The TcII/VI profile, associated with domestic cycles and patients with chronic Chagas disease, was the most prevalent among the identified DTUs. Furthermore, the correlation of the study results with complementary information made it possible to suggest that TcII is the predominant lineage of this work.

Details

Language :
English
ISSN :
1678-8060
Volume :
117
Database :
MEDLINE
Journal :
Memorias do Instituto Oswaldo Cruz
Publication Type :
Academic Journal
Accession number :
36383785
Full Text :
https://doi.org/10.1590/0074-02760220125