T cells specific for α-myosin drive immunotherapy-related myocarditis.

Immune-related adverse events, particularly severe toxicities such as myocarditis, are major challenges to the utility of immune checkpoint inhibitors (ICIs) in anticancer therapy ¹ . The pathogenesis of ICI-associated myocarditis (ICI-MC) is poorly understood. Pdcd1 ^-/- Ctla4 ^+/- mice recapitulate clinicopathological features of ICI-MC, including myocardial T cell infiltration ² . Here, using single-cell RNA and T cell receptor (TCR) sequencing of cardiac immune infiltrates from Pdcd1 ^-/- Ctla4 ^+/- mice, we identify clonal effector CD8 ⁺ T cells as the dominant cell population. Treatment with anti-CD8-depleting, but not anti-CD4-depleting, antibodies improved the survival of Pdcd1 ^-/- Ctla4 ^+/- mice. Adoptive transfer of immune cells from mice with myocarditis induced fatal myocarditis in recipients, which required CD8 ⁺ T cells. The cardiac-specific protein α-myosin, which is absent from the thymus ^3,4 , was identified as the cognate antigen source for three major histocompatibility complex class I-restricted TCRs derived from mice with fulminant myocarditis. Peripheral blood T cells from three patients with ICI-MC were expanded by α-myosin peptides. Moreover, these α-myosin-expanded T cells shared TCR clonotypes with diseased heart and skeletal muscle, which indicates that α-myosin may be a clinically important autoantigen in ICI-MC. These studies underscore the crucial role for cytotoxic CD8 ⁺ T cells, identify a candidate autoantigen in ICI-MC and yield new insights into the pathogenesis of ICI toxicity.
(© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)