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Enzymatic biotransformation of Rb3 from the leaves of Panax notoginseng to ginsenoside rd by a recombinant β-xylosidase from Thermoascus aurantiacus.
- Source :
-
World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2022 Nov 24; Vol. 39 (1), pp. 21. Date of Electronic Publication: 2022 Nov 24. - Publication Year :
- 2022
-
Abstract
- Given the important pharmacological activity of ginsenoside Rd but its low content in plants, the production of Rd by enzymatic transformation is of interest. In this study, a β-xylosidase gene Ta-XylQS from Thermoascus aurantiacus was cloned and overexpressed in Komagataella phaffii. Purified recombinant Ta-XylQS specifically hydrolyzes substrates with xylosyl residues at the optimal pH of 3.5 and temperature of 60 °C. This study established a process for producing Rd by transforming ginsenoside Rb3 in the saponins of Panax notoginseng leaves via recombinant Ta-XylQS. After 60 h, 3 g L <superscript>- 1</superscript> of Rb3 was transformed into 1.46 g L <superscript>- 1</superscript> of Rd, and the maximum yield of Rd reached 4.31 g kg <superscript>- 1</superscript> of Panax notoginseng leaves. This study is the first report of the biotransformation of ginsenoside Rb3 to Rd via a β-xylosidase, and the established process could potentially be adopted for the commercial production of Rd from Rb3.<br /> (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
- Subjects :
- Biotransformation
Plant Leaves
Panax notoginseng
Thermoascus
Subjects
Details
- Language :
- English
- ISSN :
- 1573-0972
- Volume :
- 39
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- World journal of microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 36422714
- Full Text :
- https://doi.org/10.1007/s11274-022-03472-5