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Comparison of effects of aminosalicylic acid, glucocorticoids and immunosuppressive agents on the expression of multidrug-resistant genes in ulcerative colitis.
- Source :
-
Scientific reports [Sci Rep] 2022 Nov 30; Vol. 12 (1), pp. 20656. Date of Electronic Publication: 2022 Nov 30. - Publication Year :
- 2022
-
Abstract
- To compare the effects of aminosalicylic acid, glucocorticoids and immunosuppressants on the expression levels of multidrug resistance genes in patients with ulcerative colitis (UC), with the aim of providing a theoretical and therapeutic basis for the diagnosis, treatment, and prevention of UC. Fresh colonic mucosal tissues or postoperative pathological biopsies from 148 UC patients were collected, and the distribution sites and morphology of P-glycoprotein (P-gp) were detected using immunohistochemical staining. RT-PCR was used to quantify the expression levels of multidrug resistance gene (MDR1) mRNA before and after the corresponding treatment, and the effects of aminosalicylic acid, glucocorticoids and immunosuppressive drugs on P-gp were compared. In addition, the effects of the three drugs on MDR1 mRNA were analyzed. Administration of 5-aminosalicylic acid (5-ASA) drugs did not correlate with MDR1 expression in UC, whereas administration of glucocorticoids and immunosuppressive drugs was positively correlated with MDR1 expression profile. The expression levels of MDR1 mRNA and its product P-gp were significantly upregulated in patients who did not respond to glucocorticoids and immunosuppressive drugs. 5-ASA had no effect on the expression levels of MDR1 and its product P-gp in patients with a confirmed diagnosis of UC. However, the use of glucocorticoids and immunosuppressants can increase the expression level of MDR1.<br /> (© 2022. The Author(s).)
- Subjects :
- Humans
Glucocorticoids pharmacology
Glucocorticoids therapeutic use
Immunosuppressive Agents pharmacology
Immunosuppressive Agents therapeutic use
Mesalamine pharmacology
Mesalamine therapeutic use
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
RNA, Messenger genetics
Aminosalicylic Acid
Colitis, Ulcerative drug therapy
Colitis, Ulcerative genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 36450761
- Full Text :
- https://doi.org/10.1038/s41598-022-19612-8