Molecular basis for selective activation of DREADD-based chemogenetics.

Designer receptors exclusively activated by designer drugs (DREADDs) represent a powerful chemogenetic technology for the remote control of neuronal activity and cellular signalling ^1-4 . The muscarinic receptor-based DREADDs are the most widely used chemogenetic tools in neuroscience research. The G _q -coupled DREADD (hM3Dq) is used to enhance neuronal activity, whereas the G _i/o -coupled DREADD (hM4Di) is utilized to inhibit neuronal activity ⁵ . Here we report four DREADD-related cryogenic electron microscopy high-resolution structures: a hM3Dq-miniG _q complex and a hM4Di-miniG _o complex bound to deschloroclozapine; a hM3Dq-miniG _q complex bound to clozapine-N-oxide; and a hM3R-miniG _q complex bound to iperoxo. Complemented with mutagenesis, functional and computational simulation data, our structures reveal key details of the recognition of DREADD chemogenetic actuators and the molecular basis for activation. These findings should accelerate the structure-guided discovery of next-generation chemogenetic tools.
(© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)