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FLI-1/Melan-A dual stain is an alternative to PRAME in differentiating metastatic melanoma from nodal nevus: A monocentric retrospective study.
- Source :
-
Journal of cutaneous pathology [J Cutan Pathol] 2023 Mar; Vol. 50 (3), pp. 247-258. Date of Electronic Publication: 2022 Dec 21. - Publication Year :
- 2023
-
Abstract
- Melanocytic nevi existing in lymph nodes create a diagnostic challenge by mimicking metastases. PReferentially expressed Antigen in MElanoma (PRAME) immunohistochemical (IHC) stain can differentiate one from another. FLI-1 IHC expression has been shown in malignant melanoma with variable sensitivity while melanocytic nevi were reported to be negative. We hypothesized that FLI-1/Melan-A dual IHC staining may be used in the distinction of metastatic melanoma from nodal nevi and can be an alternative and/or complementary to PRAME. In this study, we examined 13 lymph nodes with metastatic melanoma and 13 lymph nodes with benign deposits. We stained all of the lymph nodes with FLI-1, FLI-1/Melan-A dual, and PRAME IHC stains. In addition, we stained paired skin samples of the metastatic lymph nodes with FLI-1 and PRAME. In primary cutaneous melanomas, 11 of 13 were positive for FLI-1 and PRAME expression (85%). Malignant cells in 12 and 13 lymph nodes showed positive expression of PRAME and FLI-1, respectively. Only one case with a nevic cell deposit was weakly positive for FLI-1 and the remaining benign cases were negative for both FLI-1 and PRAME. Our results show that FLI-1/Melan-A dual stain is as sensitive and specific as PRAME in distinguishing lymph nodes with metastatic melanoma from nodal nevi. Further studies with larger case numbers are needed to support our significant results.<br /> (© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Humans
Antigens, Neoplasm
Biomarkers, Tumor metabolism
Coloring Agents
Lymphatic Metastasis diagnosis
MART-1 Antigen
Retrospective Studies
Sentinel Lymph Node Biopsy
Proto-Oncogene Protein c-fli-1
Melanoma, Cutaneous Malignant
Melanoma pathology
Nevus pathology
Nevus, Pigmented pathology
Skin Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0560
- Volume :
- 50
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of cutaneous pathology
- Publication Type :
- Academic Journal
- Accession number :
- 36454046
- Full Text :
- https://doi.org/10.1111/cup.14373