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Genetically engineered bacterium-modified magnetic particles assisted chiral recognition and colorimetric determination of D/L-tryptophan in millets.
- Source :
-
Food chemistry [Food Chem] 2023 May 01; Vol. 407, pp. 135125. Date of Electronic Publication: 2022 Dec 02. - Publication Year :
- 2023
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Abstract
- Chiral recognition of enantiomers has always been a thorny issue since they exhibit the same properties under an achiral environment. Herein, polydopamine-functionalized magnetic particles (MP@PDA) were synthesized to immobilize the genetically engineered bacterium Escherichia coli DH5α (MP@PDA-E. coli). L-tryptophan (Trp) instead of D-Trp can be stereo-specifically degraded by tryptophanase in E. coli. The degradation product indole reacts with 4-dimethylaminobenzaldehyde to generate a rose-red adduct. Thus, MP@PDA-E. coli was employed to fabricate a chiral colorimetric method for chiral recognition and determination of L-Trp. The method averts the purification of tryptophanase. More importantly, tryptophanase demonstrates excellent enantioselective ability for L-Trp. The method can not only quantitatively detect L-Trp but also realize the measurement of the enantiomer percentage in the enantiomeric mixture. The feasibility was verified by detecting L-Trp in millet samples from different origins. Furthermore, a portable device was fabricated to make the method more convenient.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-7072
- Volume :
- 407
- Database :
- MEDLINE
- Journal :
- Food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 36495743
- Full Text :
- https://doi.org/10.1016/j.foodchem.2022.135125