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An optical system for noninvasive microscopy of psoriatic mice in vivo.
- Source :
-
Journal of biophotonics [J Biophotonics] 2023 Apr; Vol. 16 (4), pp. e202200310. Date of Electronic Publication: 2023 Jan 02. - Publication Year :
- 2023
-
Abstract
- Psoriasis is a chronic inflammatory skin disease involved with both complex morphological changes of skin and immune processes. The clinical diagnostics and research of psoriasis often require invasive biopsy which lacks their real-time dynamics in vivo. Here we report a noninvasive microscopic system developed by combining in vivo fluorescent microscopy, optical clearing, and immunolabeling to enable real-time imaging of immune cells and cytokines in blood flow in psoriatic animal models. The vascular morphology and time-lapse kinetics of interleukin (IL)-23, IL-17, tumor necrosis factor-α, and CD4+ cells in blood are captured at submicron resolution through the thickening epidermis and opaque scales during the development of psoriasis in vivo. Our data suggest IL-23 recruits CD4+ cells to release IL-17 in blood that further leaks out in the psoriatic skin area. This optical system enables noninvasive and real-time assessment of immune molecules and cells in vivo, providing good potential for medical researches on psoriasis.<br /> (© 2022 Wiley-VCH GmbH.)
- Subjects :
- Animals
Mice
Disease Models, Animal
Interleukin-23 blood
Interleukin-17 blood
Tumor Necrosis Factor-alpha blood
CD4-Positive T-Lymphocytes immunology
Blood Vessels diagnostic imaging
Blood Vessels immunology
Psoriasis blood
Psoriasis diagnostic imaging
Psoriasis immunology
Microscopy, Fluorescence methods
Skin diagnostic imaging
Skin immunology
Optical Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1864-0648
- Volume :
- 16
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of biophotonics
- Publication Type :
- Academic Journal
- Accession number :
- 36519190
- Full Text :
- https://doi.org/10.1002/jbio.202200310