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Crystallization of a DNA duplex 15-mer containing unpaired bases: d(CGCGAAATTTACGCG).

Authors :
Miller M
Wlodawer A
Appella E
Sussman JL
Source :
Journal of molecular biology [J Mol Biol] 1987 Jun 20; Vol. 195 (4), pp. 967-8.
Publication Year :
1987

Abstract

Crystals of an almost self-complementary DNA 15-mer d(CGCGAAATTTACGCG) have been grown by the vapor diffusion technique at 4 degrees C. The space group is I222 with a = 37.3 A, b = 54.6 A and c = 104.8 A. Solution studies showed that the 15-mer forms a duplex with the extra adenine residue unpaired: (sequence; see text) Crystals are stable at 4 degrees C and are suitable for medium-resolution structural studies.

Details

Language :
English
ISSN :
0022-2836
Volume :
195
Issue :
4
Database :
MEDLINE
Journal :
Journal of molecular biology
Publication Type :
Academic Journal
Accession number :
3656440
Full Text :
https://doi.org/10.1016/0022-2836(87)90503-1