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Metabolic engineering of Aspergillus niger to enhance production of ethanol.

Authors :
de Los Santos Mondragón AI
Barragan BAA
Sánchez UR
Calleja CAL
Millán-Chiu BE
Loske AM
Lim MAG
Source :
Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2023 Jun; Vol. 70 (3), pp. 1176-1188. Date of Electronic Publication: 2023 Jan 09.
Publication Year :
2023

Abstract

This work describes the genetic transformation of a strain of Aspergillus niger with five different constructs containing 16 different heterologous genes, coding for four oxidoreductases, two cellobiohydrolases, one endoglucanase, one β-glucosidase, six enzymes involved in xylose metabolism, and two enzymes involved in fermentation. The aim was to try and engineer a consolidated bioprocessing in A. niger. The fungus already contains most of these enzymes and we only enhanced endogenous activities. We recovered nine transformants containing all genes, as indicated by polymerase chain reaction (PCR). To confirm that the products of the genes were functional, we measured the activity of five different enzymes in all the strains, and they all showed enhanced activity over the wild-type (wt) strain. The strains were grown on carboxymethyl cellulose (CMC) and xylan as substrates, and they produced considerably more ethanol than the wt. The levels of ethanol production were comparable to those reported in the literature.<br /> (© 2022 International Union of Biochemistry and Molecular Biology, Inc.)

Details

Language :
English
ISSN :
1470-8744
Volume :
70
Issue :
3
Database :
MEDLINE
Journal :
Biotechnology and applied biochemistry
Publication Type :
Academic Journal
Accession number :
36575961
Full Text :
https://doi.org/10.1002/bab.2430