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Proximity Labeling and Proteomics: Get to Know Neighbors.
- Source :
-
Methods in enzymology [Methods Enzymol] 2023; Vol. 679, pp. 131-162. Date of Electronic Publication: 2022 Sep 02. - Publication Year :
- 2023
-
Abstract
- Protein-protein interactions are essential in biological reactions and fundamental to cell-cell communication (e.g., the binding of secreted proteins, such as hormones, to cell membrane receptors) and the subsequent intracellular signal transduction cascade. Several studies have been extensively carried out on protein-protein interactions because they have the potential to resolve various problems in molecular biology. Biochemical methods, such as chemical cross-linking and immunoprecipitation, have long been used to analyze which proteins interact with each other. However, there are some problems, such as unphysiological states and non-specific binding, that require the development of more useful experimental methods. This chapter discusses the "proximity labeling (Proteomics)" analysis technique, which has been attracting attention in protein-protein interaction analysis in recent years and is used in many biological studies. "Membrane proximity labeling (proteomics)," which analyzes the interaction of cell membrane proteins, and "intracellular proximity labeling (proteomics)" will be explained in-depth.<br />Competing Interests: Conflicts of interest The authors have no competing or financial interests to declare.<br /> (Copyright © 2023 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1557-7988
- Volume :
- 679
- Database :
- MEDLINE
- Journal :
- Methods in enzymology
- Publication Type :
- Academic Journal
- Accession number :
- 36682860
- Full Text :
- https://doi.org/10.1016/bs.mie.2022.07.031