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LncRNA LINC01197 inhibited the formation of calcium oxalate-induced kidney stones by regulating miR-516b-5p/SIRT3/FOXO1 signaling pathway.
- Source :
-
Cell and tissue research [Cell Tissue Res] 2023 May; Vol. 392 (2), pp. 553-563. Date of Electronic Publication: 2023 Jan 23. - Publication Year :
- 2023
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Abstract
- Long non-coding RNA (lncRNA) plays a key role in the regulation of calcium oxalate (CaOx) crystals-induced kidney stone formation and deposition. The purpose of this study is to study the effect of lncRNA LINC01197 on CaOx-induced kidney stone formation and the underlying mechanism. Crystal cell adhesion in HK-2 cells was evaluated by analyzing Ca2+ concentration. Apoptosis was detected by flow cytometry. The RT-qPCR and western blot were used to detect the mRNA and protein expression. Patients with kidneys stones showed down-regulated LINC01197 and SIRT3 expression, and up-regulated miR-516b-5p expression. LINC01197 knockdown promoted CaOx-induced cell adherence and cell apoptosis, increased Bax, decreased Bcl-2 expression. Luciferase reporter assay showed that SIRT3 expression was promoted by LINC01197 competing binds to miR-516b-5p. In addition, LINC01197 expression was promoted by SIRT3/FOXO1 overexpression, and could be reversed by FOXO1 knockdown. In conclusion, the present study revealed that lncRNA LINC01197 inhibited CaOx-induced kidney stones formation by regulating the miR-516b-5p/SIRT3/FOXO1 signaling pathway.<br /> (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Humans
Calcium Oxalate metabolism
Signal Transduction
Apoptosis genetics
Cell Proliferation genetics
Forkhead Box Protein O1 genetics
Forkhead Box Protein O1 metabolism
RNA, Long Noncoding genetics
MicroRNAs genetics
MicroRNAs metabolism
Sirtuin 3 genetics
Sirtuin 3 metabolism
Kidney Calculi genetics
Kidney Calculi metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0878
- Volume :
- 392
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cell and tissue research
- Publication Type :
- Academic Journal
- Accession number :
- 36688989
- Full Text :
- https://doi.org/10.1007/s00441-022-03734-6