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Peripheral blood mononuclear cells contribute to myogenesis in a 3D bioengineered system of bone marrow mesenchymal stem cells and myoblasts.

Authors :
Scala P
Manzo P
Lamparelli EP
Lovecchio J
Ciardulli MC
Giudice V
Selleri C
Giordano E
Rehak L
Maffulli N
Della Porta G
Source :
Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2023 Jan 10; Vol. 10, pp. 1075715. Date of Electronic Publication: 2023 Jan 10 (Print Publication: 2022).
Publication Year :
2023

Abstract

In this work, a 3D environment obtained using fibrin scaffold and two cell populations, such as bone marrow-derived mesenchymal stem cells (BM-MSCs), and primary skeletal muscle cells (SkMs), was assembled. Peripheral blood mononuclear cells (PBMCs) fraction obtained after blood filtration with HemaTrate <superscript>®</superscript> filter was then added to the 3D culture system to explore their influence on myogenesis. The best cell ratio into a 3D fibrin hydrogel was 1:1 (BM-MSCs plus SkMs:PBMCs) when cultured in a perfusion bioreactor; indeed, excellent viability and myogenic event induction were observed. Myogenic genes were significantly overexpressed when cultured with PBMCs, such as MyoD1 of 118-fold at day 14 and Desmin 6-fold at day 21. Desmin and Myosin Heavy Chain were also detected at protein level by immunostaining along the culture. Moreover, the presence of PBMCs in 3D culture induced a significant downregulation of pro-inflammatory cytokine gene expression, such as IL6 . This smart biomimetic environment can be an excellent tool for investigation of cellular crosstalk and PBMC influence on myogenic processes.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2023 Scala, Manzo, Lamparelli, Lovecchio, Ciardulli, Giudice, Selleri, Giordano, Rehak, Maffulli and Della Porta.)

Details

Language :
English
ISSN :
2296-4185
Volume :
10
Database :
MEDLINE
Journal :
Frontiers in bioengineering and biotechnology
Publication Type :
Academic Journal
Accession number :
36704300
Full Text :
https://doi.org/10.3389/fbioe.2022.1075715