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Dual-Modal Apoptosis Assay Enabling Dynamic Visualization of ATP and Reactive Oxygen Species in Living Cells.

Authors :
Jiang L
Chen HY
He CH
Xu HB
Zhou ZR
Wu MS
Fodjo EK
He Y
Hafez ME
Qian RC
Li DW
Source :
Analytical chemistry [Anal Chem] 2023 Feb 14; Vol. 95 (6), pp. 3507-3515. Date of Electronic Publication: 2023 Feb 01.
Publication Year :
2023

Abstract

ATP and reactive oxygen species (ROS) are considered significant indicators of cell apoptosis. However, visualizing the interplay between apoptosis-related ATP and ROS is challenging. Herein, we developed a metal-organic framework (MOF)-based nanoprobe for an apoptosis assay using duplex imaging of cellular ATP and ROS. The nanoprobe was fabricated through controlled encapsulation of gold nanorods with a thin zirconium-based MOF layer, followed by modification of the ROS-responsive molecules 2-mercaptohydroquinone and 6-carboxyfluorescein-labeled ATP aptamer. The nanoprobe enables ATP and ROS visualization via fluorescence and surface-enhanced Raman spectroscopy, respectively, avoiding the mutual interference that often occurs in single-mode methods. Moreover, the dual-modal assay effectively showed dynamic imaging of ATP and ROS in cancer cells treated with various drugs, revealing their apoptosis-related pathways and interactions that differ from those under normal conditions. This study provides a method for studying the relationship between energy metabolism and redox homeostasis in cell apoptosis processes.

Details

Language :
English
ISSN :
1520-6882
Volume :
95
Issue :
6
Database :
MEDLINE
Journal :
Analytical chemistry
Publication Type :
Academic Journal
Accession number :
36724388
Full Text :
https://doi.org/10.1021/acs.analchem.2c05671