Influence of surface properties on the adhesion of bacteria onto different casings.

Bacteria adhere to the surfaces of sausage casing and form biofilms, which causes food spoilage and quality deterioration. However, bacterial adhesion to the casing surfaces has not received enough attention and has not been extensively studied. In this study, the effect of the physicochemical properties of casing surfaces on bacterial initial adhesion were investigated with Leuconostoc mesenteroides as model bacteria. The adhesion of Leuconostoc mesenteroides onto 5 types of casings were systematically investigated, including animal casings, collagen casings, cellulose casings, fiber casings, and nylon casings, which are the most frequently encountered casings in sausage processing. It was found that the number of viable cells on the casings following the trend as: animal casings > collagen casings > fiber casings > cellulose casings > nylon casings after 4 h of incubation time. This phenomenon might be due to the different physicochemical properties of the different casings. Therefore, physicochemical factors, including zeta potential, hydrophobicity and roughness of casings, zeta potential and hydrophobicity of Leuconostoc mesenteroides, were further characterized. In terms of hydrophobic interactions, the results showed that the number of bacteria attached to the casings did not conform to the trend of hydrophobic interaction. In terms of electrostatic interactions, the results showed that the number of bacteria attached to the casings did not conform to the trend of hydrophobic interaction. The casings with different surface roughnesses in a range of 1.67-20.83 μm, the variation of bacterial adhesion quantity was in good agreement with the variation trend of casing roughness, the result showed that the surface roughness was the key factor dominating the bacterial adhesion rate compared with the surface hydrophobicity and zeta potential. The results give new insights to explore the mechanism of bacterial adhesion on casings and prevent sausage spoilage.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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