Campylobacter majalis sp. nov. and Campylobacter suis sp. nov., novel Campylobacter species isolated from porcine gastrointestinal mucosa.

Strains LMG 7974 ^T and LMG 8286 ^T represent single, novel Campylobacter lineages with Campylobacter pinnipediorum and Campylobacter mucosalis as nearest phylogenomic neighbours, respectively. The results of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses of LMG 7974 ^T , LMG 8286 ^T and type strains of species of the genus Campylobacter confirmed that these strains represent novel species of the genus Campylobacter . The 16S rRNA gene sequences of both strains showed highest identity towards C. mucosalis (97.84 and 98.74 %, respectively). Strains LMG 7974 ^T and LMG 8286 ^T shared 72.5 and 73.7% ANI, respectively, with their nearest phylogenomic neighbours and less than 21 % dDDH. The draft genome sizes of LMG 7974 ^T and LMG 8286 ^T are 1 945429 bp and 1 708214 bp in length with percentage DNA G+C contents of 33.8 and 37.2 %, respectively. Anomalous biochemical characteristics and low MALDI-TOF mass spectrometry log scores supported their designation as representing novel species of the genus Campylobacte . We therefore propose to classify strain LMG 7974 ^T (=CCUG 20705 ^T ) as the type strain of the novel species Campylobacter majalis sp. nov. and strain LMG 8286 ^T (=CCUG 24193 ^T , NCTC 11879 ^T ) as the type strain of the novel species Campylobacter suis sp. nov.