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An "AND" Molecular Logic Gate as a Super-Enhancers for De Novo Designing Activatable Probe and Its Application in Atherosclerosis Imaging.
- Source :
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2023 Apr; Vol. 10 (12), pp. e2207066. Date of Electronic Publication: 2023 Feb 19. - Publication Year :
- 2023
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Abstract
- Developing activatable fluorescent probes with superlative fluorescence enhancement factor (F/F <subscript>0</subscript> ) to improve the signal-to-noise (S/N) ratio is still an urgent issue. "AND" molecular logic gates are emerging as a useful tool for enhanced probes selectivity and accuracy. Here, an "AND" logic gate is developed as super-enhancers for designing activatable probes with huge F/F <subscript>0</subscript> and S/N ratio. It utilizes lipid-droplets (LDs) as controllable background input and sets the target analyte as variable input. The fluorescence is tremendously quenching due to double locking, thus an extreme F/F <subscript>0</subscript> ratio of target analyte is obtained. Importantly, this probe can transfer to LDs after a response occurs. The target analyte can be directly visualized through the spatial location without a control group. Accordingly, a peroxynitrite (ONOO <superscript>-</superscript> ) activatable probe (CNP2-B) is de novo designed. The F/F <subscript>0</subscript> of CNP2-B achieves 2600 after reacting with ONOO <superscript>-</superscript> . Furthermore, CNP2-B can transfer from mitochondria to lipid droplets after being activated. The higher selectivity and S/N ratio of CNP2-B are obtained than commercial probe 3'-(p-hydroxyphenyl) fluorescein (HPFin vitro and in vivo. Therefore, the atherosclerotic plaques at mouse models are delineated clearly after administration with in situ CNP2-B probe gel. Such input controllable "AND" logic gate is envisioned to execute more imaging tasks.<br /> (© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)
Details
- Language :
- English
- ISSN :
- 2198-3844
- Volume :
- 10
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Advanced science (Weinheim, Baden-Wurttemberg, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 36808894
- Full Text :
- https://doi.org/10.1002/advs.202207066