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Label-free proteomic analysis and functional analysis in patients with intrauterine adhesion.
- Source :
-
Journal of proteomics [J Proteomics] 2023 Apr 15; Vol. 277, pp. 104854. Date of Electronic Publication: 2023 Feb 24. - Publication Year :
- 2023
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Abstract
- Intrauterine adhesion (IUA) is one of the principal causes of secondary infertility in women of reproductive age, which seriously affects female reproductive function and quality of life. In recent years, the incidence of IUA has been increasing year by year, but its pathological mechanism has not yet been clarified. This study intended to reveal the pathogenesis of IUA and find new therapeutic targets by analyzing the proteomic differences between intrauterine adhesion tissues and normal human endometrial tissues. In the label-free quantitative proteomics, we identified 789 up-regulated differentially expressed proteins (DEPs) and 539 down-regulated DEPs. These DEPs were further analyzed by Gene Ontology (GO) annotation and enrichment analysis, Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis to preliminarily clarify the biomarkers involved in the pathogenesis of the IUA. The DEPs were further verified by parallel reaction monitoring (PRM) to confirm the results of proteomics. Finally, 7 target proteins may be candidates for treatment and elucidating the pathophysiology of IUA. SIGNIFICANCE: IUA is a fertility complication, which has increasing incidence recently. Until now, only a little research paid attention to the proteomic changes of IUA. This is the first study focused on the comparative analysis of endometrial tissue between IUA patients and normal women. We found 7 key proteins that may become the potential biomarkers of IUA.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest.<br /> (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1876-7737
- Volume :
- 277
- Database :
- MEDLINE
- Journal :
- Journal of proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 36841354
- Full Text :
- https://doi.org/10.1016/j.jprot.2023.104854