Back to Search
Start Over
Protocol to study presynaptic protein synthesis in ex vivo mouse hippocampal slices using HaloTag technology.
- Source :
-
STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 101986. Date of Electronic Publication: 2023 Jan 19. - Publication Year :
- 2023
-
Abstract
- Presynaptic boutons in the mammalian brain are typically small and difficult to manipulate and study. Here, we present a protocol applying HaloTag self-labeling technology to detect de novo local protein synthesis in intact presynaptic mossy fiber boutons from acute mouse hippocampal slices. We describe stereotaxic injection of HaloTag-expressing virus into the brain region of interest, followed by brain slice preparation. We then detail the labeling of HaloTag-fused protein and image acquisition to visualize the labeled protein in an intact circuit. For complete details on the use and execution of this protocol, please refer to Monday et al. (2022). <superscript>1</superscript> .<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Mice
Animals
Neurons
Proteins
Mammals
Hippocampus
Presynaptic Terminals
Subjects
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 36853723
- Full Text :
- https://doi.org/10.1016/j.xpro.2022.101986