DNA labelling in live cells via visible light-induced [2+2] photocycloaddition.

Authors :: Michenfelder RT
Delafresnaye L
Truong VX
Barner-Kowollik C
Wagenknecht HA
Source :: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2023 Mar 30; Vol. 59 (27), pp. 4012-4015. Date of Electronic Publication: 2023 Mar 30.
Publication Year :: 2023
Abstract: We introduce a visible light-driven ( λ <subscript>max</subscript> = 451 nm) photo-chemical strategy for labelling of DNA in living HeLa cells via the [2+2] cycloaddition of a styrylquinoxaline moiety, which we incorporate into both the DNA and the fluorescent label. Our methodology offers advanced opportunities for the mild remote labelling of DNA in water while avoiding UV light activation.